ssr_primer
Primer Details for TRINITY_DN10024_c0_g1_i9 |
|---|
| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGAAGTCGATCGAGGGAAGG | AGGAGTTCGCCGGAAAGAAG | Start: 30 |
| Tm | 59.97 | 60.037 | End: 153 |
| Size | 20 | 20 | 124 |
| Primer 2 details |
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| Sequence | CAGGGAGAGCTGGAGCAAAA | TAACCTGACGGAGGAGGAGG | Start: 2656 |
| Tm | 59.963 | 60.033 | End: 2815 |
| Size | 20 | 20 | 160 |
| Primer 3 details |
|---|
| Sequence | GCTGGAGCAAAAGGAACTGC | TAACCTGACGGAGGAGGAGG | Start: 2664 |
| Tm | 60.039 | 60.033 | End: 2815 |
| Size | 20 | 20 | 152 |
| Primer 4 details |
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| Sequence | AATGGCCCTCAAAGGTCGAG | ACGGCAAGTTCACGAGTAGG | Start: 551 |
| Tm | 60.035 | 60.039 | End: 715 |
| Size | 20 | 20 | 165 |
| Primer 5 details |
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| Sequence | CCTACTCGTGAACTTGCCGT | TCAGCCTCATCCAGCACAAG | Start: 696 |
| Tm | 60.039 | 60.036 | End: 925 |
| Size | 20 | 20 | 230 |
(A)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGAAGTCGATCGAGGGAAGG | AGGAGTTCGCCGGAAAGAAG | Start: 30 |
| Tm | 59.97 | 60.037 | End: 153 |
| Size | 20 | 20 | 124 |
| Primer 2 details |
|---|
| Sequence | CAGGGAGAGCTGGAGCAAAA | TAACCTGACGGAGGAGGAGG | Start: 2656 |
| Tm | 59.963 | 60.033 | End: 2815 |
| Size | 20 | 20 | 160 |
| Primer 3 details |
|---|
| Sequence | GCTGGAGCAAAAGGAACTGC | TAACCTGACGGAGGAGGAGG | Start: 2664 |
| Tm | 60.039 | 60.033 | End: 2815 |
| Size | 20 | 20 | 152 |
| Primer 4 details |
|---|
| Sequence | AATGGCCCTCAAAGGTCGAG | ACGGCAAGTTCACGAGTAGG | Start: 551 |
| Tm | 60.035 | 60.039 | End: 715 |
| Size | 20 | 20 | 165 |
| Primer 5 details |
|---|
| Sequence | CCTACTCGTGAACTTGCCGT | TCAGCCTCATCCAGCACAAG | Start: 696 |
| Tm | 60.039 | 60.036 | End: 925 |
| Size | 20 | 20 | 230 |
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